DynoMOCav is advanced microfluidic platform designed to accurately model the human aortic valve. This innovative technology features a three-chamber architecture that mimics the native valve structure, with separate compartments representing the aortic side, valve matrix, and ventricular side. DynaMOC recreates the complex mechanical environment of the aortic valve by simultaneously applying physiologically relevant fluid flows and tissue stretching – specifically oscillatory flow on the aortic side and steady pulsatile flow on the ventricular side.

One of DynaMOC's key strengths is its ability to support both valve endothelial cells and valve interstitial cells in their natural spatial arrangement, while incorporating customizable hydrogel matrices composed of valve-specific components like collagen, glycosaminoglycans, and elastin. This comprehensive simulation of the valve microenvironment makes DynaMOC ideal for studying calcific aortic valve disease (CAVD), screening potential drug treatments, investigating valve mechanobiology, and developing personalized medicine approaches.

The platform offers precise control over multiple parameters including dual flow patterns, cyclic strain application, and matrix composition, allowing researchers to isolate specific factors and determine their individual contributions to valve function and disease. Fabricated from biocompatible PDMS, DynaMOC provides optimal cell viability while remaining compatible with standard microscopy and analytical techniques. By providing a more physiologically relevant alternative to conventional cell culture and reducing reliance on animal models, DynaMOC represents a significant advancement in heart valve research technology.